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Monday, July 13, 2020 | History

2 edition of Palmitylation of vaccinia virus proteins found in the catalog.

Palmitylation of vaccinia virus proteins

Douglas W. Grosenbach

Palmitylation of vaccinia virus proteins

identification of modification sites and biological relevance

by Douglas W. Grosenbach

  • 82 Want to read
  • 10 Currently reading

Published .
Written in English

    Subjects:
  • Vaccinia.,
  • Viral proteins.,
  • Post-translational modification.

  • Edition Notes

    Statementby Douglas W. Grosenbach.
    The Physical Object
    Pagination118 leaves, bound :
    Number of Pages118
    ID Numbers
    Open LibraryOL15539368M

    ined its possible role in vaccinia actin tail formation. 2. Materials and methods Cells, vaccinia virus strains and infections HeLa cells were infected with vaccinia virus strain WR as described previously [9]. HeLa cells were also infected with the recombinant vaccinia virus strain vROllk, in which expression of F17R is IPTG inducible [19]. DNA binding proteins of vaccinia virus (VV) virions, strain LIVP, have been studied by their covalent crosslinking to DNA, using two-dimensional gel retardation electrophoresis of crosslinked DNA and proteins as well as the ‘protein image’ hybridization assay.

    Vaccinia viruses encode several proteins which prevent the induction of virus-induced apoptosis. The vaccinia virus anti-apoptotic protein F1 for example was shown to inhibit Bak and Bax activation, thereby counteracting the activation of the mitochondrial pathway of apoptosis in a highly effective manner. Another vaccinia virus protein, N1. A vaccinia virus-encoded double-stranded RNA-binding protein, p25, has been previously implicated in inhibition of the interferon-induced, double-stranded RNA-activated protein kinase. In this study, we have identified the vaccinia viral gene (WR strain) that encodes pCited by:

      Orthopoxviruses are among the largest and most complex of the animal viruses. In response to the recent emergence of monkeypox in Africa and the threat of smallpox bioterrorism, two orthopoxviruses with different pathogenic potentials, human monkeypox virus and vaccinia virus, were proteomically compared with the goal of identifying proteins required for Cited by: Vaccinia E3 protein has the biochemical capacity of binding to double-stranded RNA (dsRNA). The best characterized biological functions of the E3 protein include its host range function, suppression of cytokine expression, and inhibition of interferon (IFN)-induced antiviral activity. such as poxvirus E3 family proteins, influenza virus NS1.


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Palmitylation of vaccinia virus proteins by Douglas W. Grosenbach Download PDF EPUB FB2

Vaccinia virus encodes at least eight proteins that are modified post-translationally by the addition of a carbon saturated fatty acid through thioester linkage to cysteine residues. This is referred to as palmitylation of : Douglas W.

Grosenbach. Analysis of the site occupancy constraints of primary amino acid sequences in the motif directing palmitylation of the vaccinia virus kDa envelope protein. Hansen SG(1), Grosenbach DW, Hruby DE. Author information: (1)Center for Gene ResearchBiotechnology, Oregon State University, Corvallis, Oregon,by: 7.

INTRODUCTION. Morphogenesis of the Poxviridae occurs within specialized factory regions of the cytoplasm. This process has been studied most intensively for vaccinia virus (VACV), the prototypic member of the family ().In the accompanying paper (), we show that the VACV A19 protein is required for the transition of spherical immature virions (IVs) to barrel-shaped Cited by: 6.

Resistance to human respiratory syncytial virus infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the RSV G glycoprotein.

Proc. Nail. Aced. Sci. Falkner, F. G., and Moss, B. Escherichie coli gpt gene provides dominant selection for vaccinia virus open reading frame Cited by: A mutational analysis of the vaccinia virus (VV) B5R protein is presented.

This protein is related to the regulators of complement activation (RCA) superfamily, has four short consensus repeats. @article{osti_, title = {The vaccinia virus E6 protein influences virion protein localization during virus assembly}, author = {Condit, Richard C., E-mail: [email protected] and Moussatche, Nissin}, abstractNote = {Vaccinia virus mutants in which expression of the virion core protein gene E6R is repressed are defective in virion morphogenesis.

N2 - Apoptosis is an important antiviral host defense mechanism. Here we report the identification of a novel apoptosis inhibitor encoded by the vaccinia virus (VACV) M1L gene.

M1L is absent in the attenuated modified vaccinia virus Ankara (MVA) strain of VACV, a strain that stimulates apoptosis in several types of immune by: 3. VIROLOGY() Evidence for Multiple Species of Vaccinia Virus-Encoded Palmitylated Proteins STEPHANIE J. CHILD1 AND DENNIS E. HRUBY2 Center for Gene Research and Biotechnology, Department of Microbiology, Oregon State University, Corvallis, Oregon Received accepted J When cells were Cited by: Vaccinia Virus Antibodies Vaccinia virus is a large DNA virus with a genome of ~ kb encoding open reading frames (ORFs) that is a close relative of variola virus.

Vaccinia virus morphogenesis is a complex process which occurs in the cytoplasm of infected cells and results in the formation of the intracellular mature virus (IMV) and the.

Vaccinia virus morphogenesis is a complex process which occurs in the cytoplasm of infected cells and results in the formation of the intracellular mature virus (IMV) and the intracellular enveloped virus (IEV). The IMV core contains five major protein, A3L, A4L, and A10L, while 12 proteins, A12L, A13L, A14L, AL, A17L, A27L, D8L, G4L, G7L.

The vaccinia virus I3L gene encodes a single-stranded DNA-binding protein which may play a role in viral replication and genetic recombination. We have purified native and recombinant forms of gpI3L and characterized both the DNA-binding reaction and the structural properties of DNA-protein complexes.

Vaccinia virus (VV) is the prototypical member of Orthopoxviridae, which replicates within the cytoplasm of permissive least four distinct types of enveloped infectious virus particles are produced during productive infection: mature virus, also referred to as intracellular mature virus or IMV, wrapped virus, also called intracellular enveloped virus (IEV), and two Cited by: Vaccinia virus (VV) undergoes a proteolytic processing to evolve from immature virus particles into intracellular mature virus particles.

Most of structural core protein precursors such as p4a, p4b, and p25K are assembled into previrions and then proteolytically processed to yield core proteins, 4a, 4b, and 25 K, which become components of a Cited by: 4. Molecular Genetic and Biochemical Characterization of the Vaccinia Virus I3 Protein, the Replicative Single-Stranded DNA Binding Protein Matthew D.

Greseth, Kathleen A. Boyle, Matthew S. Bluma, Bethany Unger, Matthew S. Wiebe, Jamaria A. Soares-Martins, Nadi T.

Wickramasekera, James Wahlberg, and Paula Traktman. Ralf Bartenschlager, Christa Kuhn, Heinz Schaller, Expression of the P-protein of the human hepatitis B virus in a vaccinia virus system and detection of the nucleocapsid-associated P-gene product by radiolabelling at newly introduced phosphorylation sites, Nucleic Acids Research, Vol Issue 2, 25 JanuaryPages –, https Cited by: Egress of vaccinia virus from its host cell is mediated by the microtubule-associated motor kinesin-1, and three viral proteins, A36 and the F12/E2 complex, have been implicated in this process.

Deletion of F12 expression causes a more severe reduction in egress than deletion of A36 but whether these proteins are involved in the same or Cited by: 1.

The VV genome contains numerous genes encoding proteins involved in immunomodulation and immunoevasion. For example, the virus encodes proteins that act as decoy receptors for IL-1, IL, and TNF. VV is a member of the Poxviridae, a family of complex DNA viruses that replicate in the cytoplasm of vertebrate and invertebrate cells.

Background Pre-existing immunity to Vaccinia Tian Tan virus (VTT) resulting from a large vaccination campaign against smallpox prior to the early s in China, has been a major issue for application of VTT-vector based vaccines.

It is essential to establish a sensitive and high-throughput neutralization assay to understand the epidemiology of Vaccinia-specific immunity. INTRODUCTION. Replication-competent vaccinia virus was the live poxvirus that was used as the smallpox vaccine.

The development of this vaccine was an important step in the successful eradication of smallpox, an infection characterized by fever, rash and constitutional symptoms, with a high rate of morbidity and mortality. Identification of viral-host interacting proteins will contribute to understanding of how poxvirus exploits the host cellular machinery.

The vaccinia virus gene K7R encodes a conserved protein K7 in most orthopoxviruses. To gain insight into the biology of K7, we investigated the cellular interactome of K7 by GST pulldown coupled with mass by: 5.

Palmitoylated viral proteins are well-represented in enveloped viruses, including the haemagglutinin (HA) protein of the influenza virus, Env of retroviruses and filoviruses, and F13 L of the vaccinia virus. In the vaccinia virus, palmitoylation of its F13 L protein has been shown to be essential for targeting to the appropriate by: Fuerst TR, et al.

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USAPubMed: Background: B8R. B8R is a secreted Interferon gamma (IFN-gamma) binding protein encoded by the open reading frame of the Vaccina virus (smallpox) (1, 2).

B8R is a 43 kDa protein and shares only 19% amino acid sequence identity with the extracellular region of the human IFN-gamma Receptor 1 (IFN-gamma R1).